Fermentation Technology

Okwuenu PC

Publications

• Research Article
  Effect of Divalent Metal Ions on Glucoamylase Activity of Glucoamylase Isolated from Aspergillus niger
  Author(s): Okwuenu PC, Agbo KU, Ezugwu AL, Eze SO and Chilaka FC Okwuenu PC, Agbo KU, Ezugwu AL, Eze SO and Chilaka FC
  
  This study investigates the production of glucoamylase from Aspergillus niger in a submerged fermentation process using amylopectin fractionated from guinea corn starch as the carbon source. This work also studies the effect of a few metal ions (Ca2+, Zn2+, Co2+, Fe2+, Mn2+ and Pb2+) concentration on the glucoamylase activity. A Fourteen day experimental study was carried out to determine the day of highest glucoamylase activity. Maximum glucoamylase activity was observed on day five of the submerged fermentation; hence, day five was mass produced. The specific activity for the crude enzyme was found to be 729.45 U/mg. The crude enzyme was purified to the level of gel filtration (using sephadex G-100) via ammonium sulphate precipitation. Ammonium sulphate saturation of 70% was found suitable to precipitate the enzyme. After ammonium sulphate precipitation and gel filtration, the speci.. View More»
  DOI: 10.4172/2167-7972.1000141

  Abstract PDF