Nancy Yogita Bansal
India
Research Article
HPLC-UV/FD Methods for Scopoletin and Asiatic acid: Development,
Validation and Application in WHO Recommended Stability Testing of Herbal
Drug Products
Author(s): Nancy Yogita Bansal and Gulshan BansalNancy Yogita Bansal and Gulshan Bansal
WHO recommended accelerated stability study was conducted on commercially available herbal products containing Shankhpushpi along with Bacopa monnieri (A) or Centella asiatica (B) at a temperature of 40 ± 2°C and 75 ± 5% RH for six months. Control sample of each product was stored at 4°C. Stability samples were withdrawn after 1, 3 and 6 months. Each control and stability sample was analysed for the content of scopoletin, asiatic acid and bacoside A by HPLC methods because such methods prove more selective, sensitive, efficient, reproducible, and accurate than spectroscopic and chemical methods. These methods also have advantages of sample handing, cost effectiveness and versatility in analyzing chemically diverse compounds over other methods like GSC and GLC. New methods developed and validated for scopoletin and asiatic acid were proved sufficiently precise, ac.. View More»
DOI:
10.4172/2161-1009.1000207