Journal of Plant Pathology & Microbiology

Muzafar Sheikh

Publications

• Rapid Communication
  Biological and Molecular Detection of a virus infecting Wedelia trilobata (Linn.) Hitchc (A Medicinal Herb)
  Author(s): Muzafar Sheikh, Mohd Ashraf and Irshad MahmoodMuzafar Sheikh, Mohd Ashraf and Irshad Mahmood
  
  A Potyvirus has been isolated from Wedelia trilobata plants in Aligarh district of state Uttar Pradesh (India). The infected plants were showing mosaic and mottling of leaves as shown below. On host range study virus seem to infect many species of Compositae, Umblifereae, Asteraceae, Solanaceae and Euphorbiaceae. The Thermal Inactivation Point (TIP) was between 55-60°C, dilution end point was within 10-4 and Longevity in Vitro (LIV) at 20°C was within 24 hr. The virus was transmitted by Myzus persicae (Sulzer). Cylindrical inclusion bodies were observed in tissue of the infected plants by electron microscopy. The mean length of the flexous particle was 736.5 nm. The virus was successfully purified from infected Wedelia trilobata by differential centrifugation. The purified virus showed the typical UV spectrum of a nucleoprotein with maximum and minimum absorption at 260 nm and.. View More»
  DOI: 10.4172/2157-7471.1000119

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