Fermentation Technology

Muhammad Danish

Publications

• Research Article
  Production, Purification and Partial Characterization of Organo-Solvent Tolerant Protease from Newly Isolated Bacillus sp. BBXS-2
  Author(s): Abdul Sattar Qureshi, Altaf Ahmed Simair, Chaudhry Haider Ali, Imrana Khushk, Jawaid Ahmed Khokhar, Ayyaz Ahmad, Muhammad Danish, Changrui LuAbdul Sattar Qureshi, Altaf Ahmed Simair, Chaudhry Haider Ali, Imrana Khushk, Jawaid Ahmed Khokhar, Ayyaz Ahmad, Muhammad Danish, Changrui Lu
  
  Proteolytic enzymes are applied in various industries such as detergent, leather, food, textile, cosmetics, pharmaceutical, and synthetic biotechnology. To meet commercial needs, microbial strains of high value in terms of cost-effective production have been focused. In this study, thermophilic strain, Bacillus sp. BBXS-2 was activated on simple growth medium and then transferred to Luria Bertani (LB) medium. Maximum protease concentration of 6723 U/mL under optimized fermentation conditions (molasses, corn steep liquor, pH 9.0, and 45°C). The results showed, there was no difference observed in bacterial growth and protease titer while replacing yeast extract with corn steep liquor so, in this way, about 90% cost of nitrogen source can be saved. The extracellular enzyme was purified to homogeneity from cell-free supernatant by ammonium sulphate precipitation followed by dialysis and .. View More»
  DOI: 10.4172/2167-7972.1000151

  Abstract PDF