Moradpour M
Laboratory of Plantation Crops, Institute of Tropical Agriculture,
43300 Serdang, Selangor Darulehasan
Malaysia
Research Article
Establishment of in vitro Culture of Rubber (Hevea brasiliensis) from
Field-derived Explants: Effective Role of Silver Nanoparticles in Reducing
Contamination and Browning
Author(s): Moradpour M, Aziz MA and Abdullah SNAMoradpour M, Aziz MA and Abdullah SNA
The development of in vitro methods to produce high quality clonal planting materials of c for replanting and new planting is highly essential. The establishment of in vitro cultures of challenging woody plant species like H. brasiliensis has frequently been hampered by persistent microbial contamination, phenolic production, phase change and low response to media. Therefore, the overall goal of this study was to establish an efficient in vitro propagation method for H. brasiliensis through solving those persistent problems. In determining the most suitable method of reducing explant contamination, different concentrations of sodium hypochlorite, mercuric chloride and nano silver (NS) at different immersion times were assessed on explants derived from three physiological leaf stages (bronze, light green and stable green leaf stages) of pre.. View More»
DOI:
10.4172/2157-7439.1000375