Pharmaceutica Analytica Acta

Mohammad Abdelkawy

Publications

• Research Article
  Simultaneous Determination of Salmeterol Xinafoate and Fluticasone Propionate in Bulk Powder and Seritide^® Diskus using High Performance Liquid Chromatographic and Spectrophotometric Method
  Author(s): Ahmed Samir, Hesham Salem and Mohammad AbdelkawyAhmed Samir, Hesham Salem and Mohammad Abdelkawy
  
  Five methods were developed for simultaneous determination of Salmeterol xinafoate (SAM) and Fluticasone propionate (FLU) without previous separation. In the first method (HPLC), a reversed-phase column and a mobile phase of acetonitrile: methanol (80:20 v/v) at 0.5 mLmin-1 flow rate was used to separate both drugs and UV detection at 220 nm. Linearity was obtained in concentration ranges of 50-500 μgmL-1 for Salmeterol xinafoate Fluticasone propionate. In the second method both drugs were determined using first derivative UV spectrophotometry, with zero crossing measurement at 352 and 269.5 nm for Salmeterol xinafoate and Fluticasone propionate, respectively. The third method depends on first derivative of the ratios spectra by measurements of the amplitudes at 334 and 337.5 nm for Salmeterol xinafoate and at 225 and 231.5 nm for Fluticasone propionate. Calibration graphs were est.. View More»
  DOI: 10.4172/2153-2435.1000180

  Abstract PDF