Journal of Bioequivalence & Bioavailability

Miroshnikov AE

Publications

• Research Article
  Accurate Method of HPLC-Ms/Ms Determination of Mycophenolic Acid in Human Plasma
  Author(s): Khokhlov AL, Yaichkov II, Shitov LN, Dzhurko YA, Shitova AM, Ryska M, Kubeš V, Shabrov VN and Miroshnikov AEKhokhlov AL, Yaichkov II, Shitov LN, Dzhurko YA, Shitova AM, Ryska M, Kubeš V, Shabrov VN and Miroshnikov AE
  
  A simple, accurate and precise method was developed using HPLC-MS/MS to quantify mycophenolic acid in human plasma. Mycophenolic acid-D3 was used as internal standard. Sample preparation involved protein precipitation. Chromatographic analysis was performed on a Phenomenex Kinetex C18 (30 mm × 4.6 mm, 2.6 μm) column using acetonitrile-water as the mobile phase. Detection in negative ionization mode was used to quantify mycophenolic acid by multiple reaction monitoring. The calibration curve was linear in the range of 0.5-30 μg/mL, with intra-and inter-batch values for accuracy and precision (coefficient of variation) ranging from 99.76 to 111.38% and from 2.54 to 9.01%, respectively. The method was successfully applied to conduct a pharmacokinetic study of 360 mg coated tablet "Myfortic" in 48 healthy participants; 768 blood samples w.. View More»
  

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