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Michael P Blundell
Michael P Blundell
United Kingdom
Publications
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Research Article
Feeder-Free Derivation of Functional Platelets from Human Induced Pluripotent Stem Cells
Author(s): Pei-Ju Sung, Sayandip Mukherjee, Michael P Blundell and Adrian J Thrasher Pei-Ju Sung, Sayandip Mukherjee, Michael P Blundell and Adrian J Thrasher
Derivation of patient specific induced pluripotent stem cells, in combination with directed platelet differentiation, provides a promising platform to study human thrombopoiesis or platelet disorders, and has clear potential for future clinical applications. However, efficient differentiation of human induced pluripotent stem cells (iPSCs) into functional blood cells remains challenging. In this study, we report a novel protocol for the generation of CD41a+, CD42b+, and CD61+ functional platelets from human iPSCs under feeder-free culture conditions. Platelets derived from this feeder-free system showed similar fibrinogen binding activity after agonist stimulation when compared to platelets derived from a previously reported cell line co culture system. Evidence showed these culture-derived platelets responded to different agonist stimulation by increased expression of activation mark.. View More»
DOI: 10.4172/2155-9864.1000153