Journal of Nanomedicine & Nanotechnology

Michael Leitner

Publications

• Research Article
  Molecular Addressability of Lipid Membrane Embedded Calixarenes towards Cytochrome C
  Author(s): Alexandra Poturnayova, Michael Leitner, Maja Snejdarkova, Peter Hinterdorfer, Tibor Hianik and Andreas EbnerAlexandra Poturnayova, Michael Leitner, Maja Snejdarkova, Peter Hinterdorfer, Tibor Hianik and Andreas Ebner
  
  By means of the atomic force microscopy techniques we studied the surface topography of the supported bilayer lipid membranes (sBLM) composed of 1,2-sn-glycerodimyristoylfosfatidylcholine (DMPC) with incorporated calixarenes tOct [6]CH2COOH (CX) specific to cytochrome c (cyt c). It is supposed that cyt c interacts with CX through amino groups of lysine residues at its surface. Therefore we also applied single molecule force spectroscopy (SMFS) to analyze the mechanisms of interaction of cyt c with the CX. In later case cyt c or individual NH2 group have been connected to the AFM tip through special linker. The topography of bare sBLM in a gel state (T=19ºC) revealed relatively smooth surface (RRMS=0.18 nm) and thickness ~5.1 nm which agrees well with previous studies. Incorporation of CX into DMPC bilayer resulted in increase of the surface roughness (RRMS= 0.39 nm) and in increa.. View More»
  DOI: 10.4172/2157-7439.1000202

  Abstract PDF