Journal of Nanomedicine & Nanotechnology

Mei Fen Shih

Publications

• Research Article
  Bioeffects of Transient and Low-Intensity Ultrasound on Nanoparticles for a Safe and Efficient DNA Delivery
  Author(s): Mei Fen Shih, Chung Huang Wu and Jong Yuh Cherng Mei Fen Shih, Chung Huang Wu and Jong Yuh Cherng
  
  An important advantage of polymer-based gene delivery systems over viral transfection systems is that transient gene expression without the safety concerns can be achieved. In addition to the polymeric systems to deliver DNA, therapeutic ultrasound is potentially useful because ultrasound energy can be transmitted through the body without damaging tissues and could be applied on a restricted area where the desired DNA is to be expressed. In this study, bioeffects of ultrasound on the transfection efficiency and cytotoxicity of DNA-polymer complexes on mammalian cells (HEK-293 and COS-7 cell lines) were investigated. Polymer-DNA ratios for optimal transfection efficiency and the size of PEI/DNA or PDMAEMA/DNA complexes were found not affected by ultrasound treatment. Also, electrophoresis results indicate that the tertiary DNA structure was not influenced by .. View More»
  DOI: 10.4172/2157-7439.1000276

  Abstract PDF