Journal of Plant Pathology & Microbiology

Mehdi Aghapour-ojaghkandi

Publications

• Research Article
  Visual Detection of Curly Top Virus by the Colorimetric Loop-Mediated Isothermal Amplification
  Author(s): Mohammad Amin Almasi, Mehdi Aghapour-ojaghkandi and Saeedeh AghaeiMohammad Amin Almasi, Mehdi Aghapour-ojaghkandi and Saeedeh Aghaei
  
  Curly Top Virus (CTV) in sugar beet, belonging to the family Geminiviridae, genius Curtovirusis a considerable problem in semiarid areas of the Iran and other semiarid parts of the world. There are several diagnostic methods to detect CTV, But these techniques take a long time for 3 h to tow day, requiring sophisticated tools. The aim of this study, for the first time, was to reduce the time required to detect CTV in sugar beet, using colorimetric loop-mediated isothermal amplification (LAMP) technique requiring only an ordinary water bath or thermoblock.DNA was extracted from infected naturally leaf tissues. Samples were tested for the presence of Curtovirus species by PCR and LAMP reactions to amplify replication-associated protein (rep) gene using species primers. LAMP was optimized to amplify CTV DNA under isothermal conditions by incubation at 63°C for 30 min. LAMP products w.. View More»
  DOI: 10.4172/2157-7471.1000198

  Abstract PDF