Journal of Plant Pathology & Microbiology

Mehdi Aghapour Ojaghkandi

Publications

• Research Article
  Development of Colorimetric Loop-Mediated Isothermal Amplification Assay for Rapid Detection of the Tomato Yellow Leaf Curl Virus
  Author(s): Mohammad Amin Almasi, Mehdi Aghapour Ojaghkandi, Ahmad Hemmatabadi, Fatah Hamidi and Saeedeh AghaeiMohammad Amin Almasi, Mehdi Aghapour Ojaghkandi, Ahmad Hemmatabadi, Fatah Hamidi and Saeedeh Aghaei
  
  Loop-mediated isothermal amplification (LAMP) assay is a novel technique for amplifying DNA under constant temperature, with high specificity, sensitivity, rapidity and efficiency. We applied a rapid detection protocol, for the first time, which utilizes colorimetric loop-mediated isothermal amplification for detection of Tomato Yellow Leaf Curl virus. In this regard, all four LAMP primers (i.e. F3, B3, FIP and BIP,) together with PCR primers (F and R) were designed on the basis of the SF gene of the DNA sequences of TYLCV. Even though DAS-ELISA, PCR and LAMP assays could successfully detect positive infected samples, considering the time, safety, sensitivity, cost and simplicity, the last one was overall superior. Meanwhile, among five different visual dyes to accurately detect LAMP products, both hydroxynaphthol blue and GeneFinderTM could produce long stable color change and bright.. View More»
  DOI: 10.4172/2157-7471.1000153

  Abstract PDF