Journal of Stem Cell Research & Therapy

María Luisa Hernández-Bule

Publications

• Research Article
  Chondrogenic Differentiation of Adipose-Derived Stem Cells by Radiofrequency Electric Stimulation
  Author(s): María Luisa Hernández-Bule, María Ángeles Trillo, María Ángeles Martínez-García, Carlos Abilahoud and Alejandro Úbeda María Luisa Hernández-Bule, María Ángeles Trillo, María Ángeles Martínez-García, Carlos Abilahoud and Alejandro Úbeda
  
  Objective: Although capacitive-resistive electric transfer (CRET) therapies, based on transdermal application of electrothermal radiofrequency currents, have shown promising therapeutic effectiveness in regeneration of traumatic or degenerative tissue lesions, their potential effects on tissues like cartilage, having poor regenerative capabilities, have not been studied sufficiently. Here we investigate the effects of the exposure to a 448 kHz current typically used in CRET therapy, on the early chondrogenic differentiation of human, adipose-derived stem cells (ADSC). Materials and methods: Stem cells obtained from healthy donors were differentiated in chondrogenic medium for 16 days. During the last 2 days of incubation the cultures were intermittently exposed or sham-exposed to a 448- kHz, sine wave current, administered at a 50 μA/mm.. View More»
  DOI: 10.4172/2157-7633.1000407

  Abstract PDF