Malini Kotak
India
Research Article
New Method for Isolation of Plant Probiotic Fluorescent Pseudomonad and Characterization for 2,4-Diacetylphluoroglucinol Production under
Different Carbon Sources and Phosphate Levels
Author(s): Sumant Chaubey, Malini Kotak and Archana G
Sumant Chaubey, Malini Kotak and Archana G
Aims: Present work describes the new enrichment method for the isolation of effective root colonizing and rhizospheric competent strains of genus fluorescent Pseudomonad and study of metabolic regulation of 2,4-DAPG biosynthesis in them under carbon sources and Pi levels.
Methods and Results: Three rounds of plant assay was performed using root tip attached microorganism mixtures for the next round of root treatment followed by phonotypical separation of fluorescent colonies to isolate fluorescent pseudomonad strains from different crop and vegetables rhizospheres. Isolated strains were characterized for their Plant Growth Promoting Rhizobacteria (PGPR) traits viz phosphate solubilisation, production of siderophore, IAA, HCN, 1-aminocyclopropane-1-carboxylate /L-methionine utilization pathway and antifungal metabolites produ.. View More»
DOI:
10.4172/2157-7471.1000253