Mahya Mozaffarzogh
Science and Research Branch, Tehran
West Indies
Research Article
Determining the Presence of Panton-Valentine Leukocidin PVL Virulence Gene
and Methicillin-Resistant Gene mecA in Staphylococcus aureus Strains Isolated
from Cream Puffs Sold by Confectionaries in Tehran by Multiplex PCR and
Studying their Antibiotic Resistance
Author(s): Mahya Mozaffarzogh and Hadis Khaleghnezhad
Mahya Mozaffarzogh and Hadis Khaleghnezhad
Staphylococcus aureus is a major cause of food poisoning in the world that is created by consumption of contaminated food. Resistance to a variety of common and specific antibiotics is increasing. Staphylococcus aureus including PVL and gene mecA to heat pasteurization and many proteolytic enzymes are stable and can remain active for a long time in food samples. The purpose of this study was to isolate Staphylococcus aureus and identify virulence gene PVL and gene of methicillin resistance in bread pastry cream by multiplex PCR technique has been used. The study included 50 samples of bread pastry cream collected 23 cases (49%) Staphylococcus aureus isolates were detected. Antibiotic susceptibility testing by disk diffusion method according to CLSI guidelines was conducted. To identify and confirm Staphylococcus aureus.. View More»