Journal of Microbial & Biochemical Technology

Mahmoud S Helwa

Publications

• Research Article
  Identification of Trichoderma spp. by DNA Barcode and Screening for Cellulolytic Activity
  Author(s): Abdelmegid I Fahmi, Ragaa A Eissa, Khalil A El-Halfawi, Hanafy A Hamza and Mahmoud S Helwa Abdelmegid I Fahmi, Ragaa A Eissa, Khalil A El-Halfawi, Hanafy A Hamza and Mahmoud S Helwa
  
  Species identification of isolates of Trichoderma from different locations of Nile delta of Egypt was performed and their cellulolytic activities were analyzed. On the basis of morphological characteristics, 75% of isolates were identified to species level and they were divided into four aggregate groups. Morphological characterization alone was insufficient to precisely identify Trichoderma species because they have relatively few morphological characters and limited variation that cause overlapping and misidentification of the isolates. Therefore, there was a necessity to use a molecular technique to compensate for the limitations of morphological characterization. The DNA sequencing of the 5.8S-ITS region was carried out using specific primers ITS1 and ITS4. By comparing the sequences of the 5.8S-ITS region to the sequences deposited in GenBank using BLAST program all isolates can .. View More»
  DOI: 10.4172/1948-5948.1000286

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