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Maha A El Demellawy
Maha A El Demellawy
Egypt
Publications
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Research Article
In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells
Author(s): Marwa M Abu-Serie, Maha A El Demellawy, Mohamed El-Sayed and Fatma El-Rashidy Marwa M Abu-Serie, Maha A El Demellawy, Mohamed El-Sayed and Fatma El-Rashidy
Colonic epithelium composes of various cell types including alkaline phosphate-expressing absorptive, mucussecreting goblet and neuroendocrine cells that are derived from stem cells through asymmetric division. The continuous renewal of stem cells occurs under the highly coordinated cellular redox state. In the current study, based on a comparison with other culture media, colon epithelial cells were able to be sustained in vitro with normal status for more than two months under the chosen culture condition; α-MEM medium containing 20% fetal bovine serum. The cultured epithelial cells had normal doubling time and normal morphological characteristics as examined by transmission electron microscope. Also, these cultured cells contained functional stem cells and maintained their differentiation potency of colon stem cells, compared with freshly isolated mucosal epithelial cells, as.. View More»
DOI: 10.4172/2161-1009.1000239