Journal of Plant Pathology & Microbiology

Luitgardis Seigner

Publications

• Research Article
  Detection, Identification and Quantification of Fusarium graminearum and Fusarium culmorum in Wheat Kernels by PCR Techniques
  Author(s): Rabab Sanoubar, Astrid Bauer and Luitgardis Seigner Rabab Sanoubar, Astrid Bauer and Luitgardis Seigner
  
  This study was carried out on 172 samples of winter wheat. The samples consisted of various cultivars that had been randomly collected from farmers’ fields in different areas of Bavaria, South Germany. The objectives of this study were detecting the presence of Tri-5 gene producing fungus that generates trichothecene mycotoxins, especially Deoxynivalenol (DON), by using conventional qualitative PCR; determining the correlation between the presence of Tri-5 gene and DON content; evaluating the Fusarium graminearum and Fusarium culmorum infection by Real-Time PCR and estimating the correlation between DON content and the severity of F. graminearum and F. culmorum contamination. This study showed that 86% of all infected samples had a Tri-5 gene and amplified a single 544bp fragment associated with a detectable amount of DON (ranged from 10 to 2990 μg kg-1). Thi.. View More»
  DOI: 10.4172/2157-7471.1000287

  Abstract PDF