Journal of Microbial & Biochemical Technology

Luis E Trujillo

Publications

• Research Article
  Kinetics of Sucrose Hydrolysis by Immobilized Recombinant Pichia pastoris Cells in a Batch reactors
  Author(s): Duniesky Martínez, Carmen Menendez, Felix M Echemendia, Lazaro Hernandez, Alina Sobrino, Luis E Trujillo, Ivan Rodriguez and Enrique R PerezDuniesky Martínez, Carmen Menendez, Felix M Echemendia, Lazaro Hernandez, Alina Sobrino, Luis E Trujillo, Ivan Rodriguez and Enrique R Perez
  
  Sucrose hydrolysis was carried out in a constant-volume batch reactor, using recombinant Pichia pastoris BfrA4X whole cells expressing Thermotoga maritima invertase, entrapped in calcium alginate beads. The kinetics of the enzymatic hydrolysis of sucrose by the biocatalyst was examined at substrate concentrations ranging between 0.03 M and 2.04 M. The reaction rate increases until 0.31 M after which the reaction velocity was constant until 1.16 M, above this concentration, the reaction rate decreases with increasing sucrose concentration. The experimental data obtained with two weight of the biocatalyst were incorporated into two kinetic models to predict the reaction time needed for sucrose hydrolysis. One model was applied for sucrose concentrations bellow 1.16 M while a second one could be used at inhibitory range between 1.46 and 2.04 M with a k value as function of initial sucros.. View More»
  DOI: 10.4172/1948-5948.1000228

  Abstract PDF