Biochemistry & Analytical Biochemistry

Lucia Calleja

Publications

• Research Article
  Remodelling of Lipid Rafts during In vitro Capacitation and Acrosome Reaction of Ram Spermatozoa
  Author(s): Carmen Colas, Rosaura Perez-Pe, Adriana Casao, Mario Ollero, Lucia Calleja, Margarita Gallego, Teresa Muiño-Blanco and Jose A Cebrian-PerezCarmen Colas, Rosaura Perez-Pe, Adriana Casao, Mario Ollero, Lucia Calleja, Margarita Gallego, Teresa Muiño-Blanco and Jose A Cebrian-Perez
  
  Background: Lipid rafts are often known as Detergent-Resistant Microdomains (DRMs). We report for the first time the presence of two lipid raft markers, caveolin-1 and ganglioside GM1, on the ram sperm surface, and the effect of in vitro capacitation and acrosome reaction on these marker distributions, the protein content and lipid composition of DRM and non-DRM fractions. Methods: Caveolin-1 and ganglioside GM1 were evidenced by immunocytochemical and fluorescence analysis, respectively. DRM and non-DRM fractions were separated by an OptiPrepTM density gradient. Cholesterol by fluorometry, GM1 by peroxidase reaction, protein content by spectrophotometry, and fatty acid profiling by gas chromatography were determined. Results: Caveolin-1 was evidenced at the acrosome of 59.2 ± 4.3% fresh spermatozoa, and the proportion of stained cells increased .. View More»
  DOI: 10.4172/2161-1009.S5-001

  Abstract PDF