Journal of Microbial & Biochemical Technology

Lisa J. Van Houten

Publications

• Research Article
  Comparison of Antibodies Raised Against Heat- and Gamma Radiation-Killed Bacteria
  Author(s): Andrew G. Gehring, Glenn Boyd, Jeffrey D. Brewster, Peter L. Irwin, Donald W. Thayer and Lisa J. Van HoutenAndrew G. Gehring, Glenn Boyd, Jeffrey D. Brewster, Peter L. Irwin, Donald W. Thayer and Lisa J. Van Houten
  
  For antibody generation, pathogenic bacteria are often heat-treated prior to inoculation into host animals in order to prevent infection and subsequently, premature death of the host. Inoculation of host rabbits with gamma radiationkilled pathogenic bacteria was employed with the hopes of generating antibodies that would have higher affinity, relative to antibodies raised against thermally denatured microorganisms, to live pathogens. The two antibody sets, raised against either heat-killed or irradiated bacterial cells, were compared for immunological response with live, heattreated, chemically-treated (i.e., bleached), and irradiated Escherichia coli O157:H7 and Salmonella bacteria. With the exception of the chemically-treated cell immunological response, both antibody sets yielded similar responses— low for irradiated cells, moderate for live cells, and high for heat-treated c.. View More»
  DOI: 10.4172/1948-5948.S2-004

  Abstract PDF