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Li Z
Li Z
China
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Research Article
Effect of Electrical Stimulation at Different Time on Mitochondrial Function of C2C12 Myotubes
Author(s): Dong HL, WU HY, Zhao J, Huang YW, Li Z, Zhang YH and Xu XYDong HL, WU HY, Zhao J, Huang YW, Li Z, Zhang YH and Xu XY
Objective: To study the effect of different time of electrical stimulation on mitochondrial function of C2C12 myotubes, and further explore its molecular mechanism. Methods: An electrical stimulation was given 7 days after C2C12 myotubess differentiation, of which intensity was 30 ms, 3Hz, and the stimulation time was 60 mins, 120 mins, and 180 mins, respectively. A total of four experimental 4 groups, including control group (Con), 60 mins group (E60), 120 mins (E120) and 180 mins (E180). Microscope was used to observe the muscular myotubes form; Kits were to detect MDA, SOD and ROS; Western blot was used to detect the expression of autophagy proteins and mechanism proteins, including PGC1, p-ULK, SIRT1 and SIRT3; Flow cytometry technology was used to detect muscle mitochondrial membrane potential. Results: There was no significant difference of C2C12 myotubes form after different elect.. View More»
DOI: 10.4172/2167-7956.1000162