Journal of Aquaculture Research & Development

Laura M Hawley

Publications

• Research Article
  Use of Ultraviolet C (UVC) Radiation to Inactivate Infectious Hematopoietic Necrosis Virus (IHNV) and Viral Hemorrhagic Septicemia Virus (VHSV) in Fish Processing Plant Effluent
  Author(s): Luis OB Afonso *,Zina Richmond ,Alexandra A Eaves ,Jon Richard ,Laura M Hawley ,Kyle A Garver Luis OB Afonso *,Zina Richmond ,Alexandra A Eaves ,Jon Richard ,Laura M Hawley ,Kyle A Garver
  
  We determined the stability of infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV) suspended in either fish processing plant effluent blood water (EBW) or culture media and examined the effectiveness of UVC radiation to inactivate IHNV and VHSV suspended in both solutions. Without exposure to UVC, IHNV and VHSV were maintained in 4°C blood water for up to 48 hours without significant reduction in virus titer. However when exposed to UVC radiation using a low pressure mercury vapour lamp collimated beam, IHNV and VHSV were inactivated, and the efficacy of UVC radiation was dependent upon the solution and virus type being treated. A 3-log reduction for VHSV and IHNV in culture media was achieved at 3.28 and 3.84 mJ cm -2 , respectively. The UV dose needed for a 3-log reduction of VHSV in EBW was 3.82 mJ cm -2 . However, exposure of IH.. View More»
  DOI: 10.4172/2155-9546.1000120

  Abstract PDF