Ladaporn Bodhidatta
Thailand
Research
Distribution and Molecular Characterization of Enteroaggregative Escherichia coli Isolated from Children in A Case-control Study of Acute Diarrhea in Thailand
Author(s): Oralak Serichantalergs, Ladaporn Bodhidatta, Panida Nobthai, Sirigade Ruekit, Krongkaew Supawat, Brett E Swierczewski and Carl J MasonOralak Serichantalergs, Ladaporn Bodhidatta, Panida Nobthai, Sirigade Ruekit, Krongkaew Supawat, Brett E Swierczewski and Carl J Mason
Background: Enteroaggregative Escherichia coli (EAEC) is a major cause of acute diarrhea in children, adults, and travelers as well as an important cause of persistent diarrhea. Due to the limited information on EAEC virulence genes, EAEC isolates isolated from children with diarrhea and asymptomatic controls in Thailand were characterized for the presence of virulence factors.
Method: A study of diarrhea etiology in children from Thailand was conducted during 2008-2009 and 301 EAEC isolates were identified by pCVD432 probe/hybridization assays from diarrhea cases and controls. A total of 200 EAEC isolates were further characterized by PCR for EAEC genotypes, aggregative adherence fimbria (AAF/I-AAF/ IV), serine protease autotransporters of Enterobacteriaceae (SPATEs) class I & II, and enterotoxin genes.
Results: T.. View More»
DOI:
10.4172/2327-5073.1000297