Fermentation Technology

KPJ Hemalatha

Publications

• Research Article
  Production of Alkaline Protease from Bacillus licheniformis through Statistical Optimization of Growth Media by Response Surface Methodology
  Author(s): BKM Lakshmi and KPJ Hemalatha BKM Lakshmi and KPJ Hemalatha
  
  An alkaline protease producing bacterial strain Bacillus licheniformis (MTCC NO. 7053), which was obtained from IMTECH, Chandigarh used for protease production studies. Three significant variables effecting protease production like rice husk (variable 1); inoculum size (variable 2); KNO3 (variable 3) have been identified under preliminary optimization studies. These variables are selected for alkaline protease production in current study. By using these eloquent variables, the basal media was subjected to statistical optimization using response surface methodology. The superlative growth media components and bacterial growth conditions for ultimate protease production were as follows: rice husk (3%); inoculum size (2%); KNO3 (0.75%); salt solution, 5% (v/v)-{(MgSO4.7H2O, 0.5% (w/v); KH2PO4 0.5% (w/v)}; and FeSO4.7H2O, 0.01% (w/v) at 37°C and 160 rpm for 72 h production. The alakal.. View More»
  DOI: 10.4172/2167-7972.1000130

  Abstract PDF