Journal of Stem Cell Research & Therapy

Kosuke Saito

Publications

• Research Article
  Functional Nerve-Vascular Reconstitution of the Bladder-Wall; Application of Patch Transplantation of Skeletal Muscle-Derived Multipotent Stem Cell Sheet-Pellets
  Author(s): Shuichi Soeda, Tetsuro Tamaki, Hiroyuki Hashimoto, Kosuke Saito, Akihiro Sakai, Nobuyuki Nakajima, Kenei Nakazato, Maki Masuda and Toshiro Terachi Shuichi Soeda, Tetsuro Tamaki, Hiroyuki Hashimoto, Kosuke Saito, Akihiro Sakai, Nobuyuki Nakajima, Kenei Nakazato, Maki Masuda and Toshiro Terachi
  
  A three-dimensional gel-patch-like nerve-vascular reconstitution system using the Skeletal Muscle-Derived Multipotent Stem Cell (Sk-MSC) sheet-pellet was applied to the reconstitution of the severely damaged bladder wall as a non-skeletal muscle tissue, but has high demand for function. The Sk-MSC sheet-pellet was prepared by the mild detachment of expanded/confluent cells in culture with EDTA, then, collected in a tube and centrifuged. The sheetpellet was pasted on the open thin-walled region of the damaged bladder wall made by myotomy (remove one-third of serosal smooth muscle layer associate with large disruptions of nerve-blood vessel networks retaining the mucosal layer). At 4 wk after transplantation, significant prevention of the reduction in the passive wall-tension, and the positive wall-contraction via electrical stimulation was observed in .. View More»
  DOI: 10.4172/2157-7633.1000142

  Abstract PDF