Journal of Stem Cell Research & Therapy

Kimitoshi Nakamura

Publications

• Research Article
  Expression Sites of Neural Stem Cell-Related Genes in the Monkey Retina
  Author(s): Maho Shibata, Tsunehiko Ikeda, Kimitoshi Nakamura, Keigo Kakurai, Seita Morishita, Masanori Fukumoto, Teruyo Kida, Taeko Horie and Hidehiro Oku Maho Shibata, Tsunehiko Ikeda, Kimitoshi Nakamura, Keigo Kakurai, Seita Morishita, Masanori Fukumoto, Teruyo Kida, Taeko Horie and Hidehiro Oku
  
  Objectives: Based on the hypothesis that undifferentiated retinal stem cell (RSC)-like cells exist in the fovea (the light-stressed, concave, avascular center of the retina where light is focused), we investigated the expression sites of neural stem cell (NSC)-related genes in the monkey retina. Methods: Cynomolgus monkeys were euthanized, and both eyes were then enucleated. Each eye was hemisected near the limbus, and flat-mounted retina samples were then prepared. Using a stereomicroscope, 1-mm x 1-mm blocks of the retina at the fovea, mid-periphery, and extreme periphery were then excised. These samples were used for real-time polymerase chain reaction analysis of the NSC-related gene (nestin, PAX6, and SOX2) expression at each site. Results: Nestin expression was high in the fovea, with a lower expression in the mid-.. View More»
  DOI: 10.4172/2157-7633.1000319

  Abstract PDF