Journal of Bacteriology & Parasitology

Kimberly R. Boer

Publications

• Research Article
  Establishment of Valid Laboratory Case Definition for Human Leptospirosis
  Author(s): Marga G.A. Goris, Mariska M.G. Leeflang, Kimberly R. Boer, Marco Goeijenbier, Eric C.M. van Gorp, Jiri F.P. Wagenaar and Rudy A. HartskeerlMarga G.A. Goris, Mariska M.G. Leeflang, Kimberly R. Boer, Marco Goeijenbier, Eric C.M. van Gorp, Jiri F.P. Wagenaar and Rudy A. Hartskeerl
  
  Laboratory case definition of leptospirosis is scarcely defined by a solid evaluation that determines cut-off values in the tests that are applied. This study describes the process of determining optimal cut-off titers of laboratory tests for leptospirosis for a valid case definition of leptospirosis. In this case the tests are the microscopic agglutination test (MAT) and an in-house IgM enzyme-linked immunosorbent assay (ELISA) both on single serum and paired samples using a positive culture as the reference test in the Dutch population. The specificity was assessed using panels of sera from healthy donors, cases with known other diseases and non-leptospirosis cases with symptoms compatible with leptospirosis. Cases were divided into three periods corroborating the acute phase (1-10 days post onset of illness (DPO)), the early convalescent (11-20 DPO) and the late convalescent phase .. View More»
  DOI: 10.4172/2155-9597.1000132

  Abstract PDF