Journal of Stem Cell Research & Therapy

Kim Bergström

Publications

• Research Article
  In vivo Effects of Bioactive Glass S53P4 or Beta Tricalcium Phosphate on Osteogenic Differentiation of Human Adipose Stem Cells after Incubation with BMP-2
  Author(s): Martin Waselau, Mimmi Patrikoski, Bettina Mannerström, Mari Raki, Kim Bergström, Brigitte von Rechenberg and Susanna Miettinen Martin Waselau, Mimmi Patrikoski, Bettina Mannerström, Mari Raki, Kim Bergström, Brigitte von Rechenberg and Susanna Miettinen
  
  Aims: Human adipose stem cells (hASCs) have been suggested as viable alternative for bone tissue engineering. However, the tissue response and osteogenic potential of BAG S53P4 or β-TCP granules has not been studied in vivo when seeded with hASCs and/or co-incubated with BMP-2 and thus, was evaluated in the current study. Methods and results: Human ASCs were isolated, expanded and seeded on BAG and β-TCP in vitro and, cell viability was assessed using Live/Dead staining. In a subcutaneous rodent implantation model, the cellular response and osteogenic potential of 1) plain, 2) hASC seeded, 3) BMP-2 co-incubated and 4) hASC seeded and BMP-2 co-incubated BAG and β-TCP granules were investigated using computed tomography and semi-quantitative histologic scores after 4 and 8 weeks. Live/Dead .. View More»
  DOI: 10.4172/2157-7633.1000125

  Abstract PDF