Journal of Nanomedicine & Nanotechnology

Keng-Liang Ou

Publications

• Research Article
  Probing Real-Time Response to Multitargeted Tyrosine Kinase Inhibitor 4-N-(3′-Bromo-Phenyl) Amino-6, 7-Dimethoxyquinazoline in Single Living Cells Using Biofuntionalized Quantum Dots
  Author(s): May-Show Chen, Chia-Yeh Liu, Wei-Ting Wang, Chien-Ting Hsu, Chih-Ming Cheng, Jing-Shin Tsai, Keng-Liang Ou and Tzu-Sen YangMay-Show Chen, Chia-Yeh Liu, Wei-Ting Wang, Chien-Ting Hsu, Chih-Ming Cheng, Jing-Shin Tsai, Keng-Liang Ou and Tzu-Sen Yang
  
  Recently, the quinazolinederivative, 4- N -(3′-bromo-phenyl) amino-6, 7-dimethoxyquinazoline (PD153035), has been reported not only to inhibit the epidermal growth factor receptor (EGFR) tyrosine kinase but also to bind to DNA double helical structures by intercalation . However, several important pharmacology issues such as whether PD153035 is a specific and reversible inhibitor of the EGFR tyrosine kinase should be addressed in more detail. In this study, we propose a nanotechnology-based approach to monitoring the real-time EGF-EGFR complex trafficking process and its relationship to cytoskeleton, as well as spatio-temporal cellular response to PD153035 at the single-cell level. W e utilize the biofunctionalized quntum dots (QDs) conjugated with EGF to monitor the cellular distribution of QD-EGF-EGFR complexes, which can provide a more .. View More»
  DOI: 10.4172/2157-7439.1000117

  Abstract PDF