Journal of Aquaculture Research & Development

Kari Ruohonen

Publications

• Research Article
  Probiotic and Pathogen Ex-vivo Exposure of Atlantic Salmon (Salmo Salar L.) Intestine from Fish Fed Four Different Protein Sources
  Author(s): Mali Hartviksen, Jose L Gonzalez Vecino, Anu Kettunen, Reidar Myklebust, Kari Ruohonen, Simon Wadsworth and Einar RingøMali Hartviksen, Jose L Gonzalez Vecino, Anu Kettunen, Reidar Myklebust, Kari Ruohonen, Simon Wadsworth and Einar Ringø
  
  The present study addressed the adherence of Carnobacterium divergens and Aeromonas salmonicida subsp. salmonicida to the intestinal lining of Atlantic salmon (Salmo salar L.) using an ex vivo method–intestinal sacfollowing feeding with pea protein concentrate, extracted sunflower or feather meal at 200 g/kg inclusion level. Control diet was a 450 g/kg fishmeal diet. The experimental feeds were fed to two groups each for seven weeks at EWOS Innovations’ facilities in Lønningdal, Norway. Ex vivo intestinal challenge was carried out at Institute for Marine Research, Bergen. Excised intestines of salmon from all feeding groups were exposed to a probiotic, C. divergens or a pathogen, A. salmonicida either alone or in combination and control samples were exposed to sterile saline solution. Exposure to A. salmonicida caused severe damage to the intestinal ultrastructure o.. View More»
  DOI: 10.4172/2155-9546.1000340

  Abstract PDF