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Kaisheng Lai
Kaisheng Lai
China
Publications
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Research Article
Characteristics of OXA-48-Producing Escherichia coli, Enterobacter cloacae and Klebsiella oxytoca from a Chinese Hospital
Author(s): Qiang Zhao, Feng Wang, Kaisheng Lai, Yanning Ma and Jiyong Yang Qiang Zhao, Feng Wang, Kaisheng Lai, Yanning Ma and Jiyong Yang
Objectives: The OXA-48-like enzymes are mainly produced by various enterobacteriaceae. The aim of this study was to investigate the characteristics of OXA-48-producing Escherichia coli, Enterobacter cloacae and Klebsiella oxytoca from China. Methods: Carbapenemase genes (blaVIM, blaOXA-48, blaKPC, blaIMP and blaNDM) were screened by PCR and subsequent amplicons sequencing. Genetic relationship was investigated by pulsed field gel electrophoresis and multilocus sequence typing. The size and incompatibility types of blaOXA-48-carrying plasmids were analyzed by the S1-PFGE, Southern blot and multiple PCR. Results: Several OXA-48-producing enterobacterial isolates were collected, including three E. coli, three E.. View More»
DOI: 10.4172/2327-5073.1000320