Journal of Nanomedicine & Nanotechnology

Kai Griebenow

Publications

• Short Communication
  Development of HAMLET-like Cytochrome c-Oleic Acid Nanoparticles for Cancer Therapy
  Author(s): Yamixa Delgado, Moraima Morales-Cruz, José Hernández-Román, Glinda Hernández and Kai Griebenow Yamixa Delgado, Moraima Morales-Cruz, José Hernández-Román, Glinda Hernández and Kai Griebenow
  
  In the literature, HAMLET (Human Alpha-lactalbumin Made LEthal to Tumor cells) is described as a tumoricidal complex composed of oleic acid (OA) non-covalently bound to α-lactalbumin. We recently demonstrated that OA is the real drug in this complex and that the protein solely serves as the drug carrier. We hypothesized that by replacing α-lactalbumin with a bioactive protein it should be possible to synergistically increase the efficiency of the complex. Consequently, we developed a HAMLET-like complex composed of OA coupled to the apoptosis-inducing protein cytochrome c (Cyt c). As control we coupled OA to the non-toxic protein bovine serum albumin (BSA). The syntheses of HAMLET-like Cyt c-OA and BSA-OA complexes micelles were performed at pH 8 and 45°C and we loaded 10 and 53 molecules of OA per molecule of Cyt c and BSA, respective.. View More»
  DOI: 10.4172/2157-7439.1000303

  Abstract PDF