Jyoti P Jadhav
Professor and Head, Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur 416004,
India
Research Article
Single Cell Protein Production Using Penicillium ochrochloron Chitinase
and Its Evaluation in Fish Meal Formulations
Author(s): Nilambari S Patil and Jyoti P JadhavNilambari S Patil and Jyoti P Jadhav
The conversion of the enzymatic hydrolysate of shellfish chitin to single-cell protein was investigated. The endproduct of chitin hydrolysis by Penicillium ochrochloron chitinase was mainly N-acetyl-D-glucosamine, its further utilization as a substrate for SCP production using Yerrowia lipolytica NCIM 3450 has been studied. The 2% chitin hydrolysate was found to be optimal for SCP production. A biomass of 9.4 g/l, total protein and nucleic acid content of the biomass was 65% and 2.9%, respectively. Fish diets were formulated to replace fishmeal partially by single cell protein from Yerrowia lipolytica using chitin hydrolysate in diets of Lepidocephalus thermalis for a period of 2 weeks. Control diet and three experimental diets were prepared to replace 25%, 50% and 75% of fish meal using SCP. The result indicated 50% yeast SCP diet gave the better growth response in Lepidocephalus the.. View More»
DOI:
10.4172/1948-5948.S4-005