Journal of Aquaculture Research & Development

Jen-Leih Wu

Publications

• Research Article
  The Liver-Enriched Transcription Factors HNF-1α, HNF-3β, and C/ EBPβ Contribute to the Growth Hormone-Induced Transcription of the Progranulin a Gene in Zebrafish (Danio Rerio)
  Author(s): Yi-Pei Lin,Wen-Jen Chung,Jen-Leih Wu*,Shao-Yang Hu*Yi-Pei Lin,Wen-Jen Chung,Jen-Leih Wu*,Shao-Yang Hu*
  
  Progranulin (PGRN) is a secreted growth factor that has been implicated in diverse biological processes including wound healing, embryo development, morphogenesis and disease. We previously showed that PGRN is induced along with IGF-1 in tilapia liver upon GH administration. In the present study, we demonstrate that the coinduction of PGRN and IGF-1 by GH administration is a common regulation in fish also presented in zebrafish. To understand the regulatory mechanism of GH-induced PGRN expression, the zebrafish PGRN promoter was isolated and analyzed. We found that a region from -2400 to -3000 within PGRN promoter is essential for GH-induced PGRN expression. A promoter competition assay showed that HNF-3 β, HNF-1 α and C/EBP β binding motifs within the -2400/-3000 region of PGRN promoter contributed to GH-induced PGRN expression. The individual or concomitant tr.. View More»
  DOI: 10.4172/2155-9546.1000237

  Abstract PDF