Journal of Liver

Jeffrey A Pearl

Publications

• Research Article
  Fibroblast Growth Factor 19 Activates the Unfolded Protein Response and Mitogen-Activated Protein Kinase Phosphorylation in H-69 Cholangiocyte Cells
  Author(s): Hannan A Qureshi, Jeffrey A Pearl, Kristy A Anderson and Richard M Green Hannan A Qureshi, Jeffrey A Pearl, Kristy A Anderson and Richard M Green
  
  Objective: Cholangiocytes are injured in many cholestatic diseases that can progress to cirrhosis, liver cancer and need for liver transplantation. Recent studies demonstrate that the hormone Fibroblast Growth Factor 19 (FGF19) is produced in the ileum and regulates hepatic gene expression. However, the role of FGF19 in cholangiocytes remains largely unknown. The purpose of this study was to elucidate the effect of FGF19 on cholangiocyte gene and protein signaling. Methods: Human cholangiocyte-derived H-69 cells were cultured and treated with varying concentrations of FGF19 (0–50 ng/ml) for 24 hours. Expression of the mitogen-activated protein kinase MAPK proteins JNK1/2, ERK1/2, and p38, and several Unfolder Protein Response (UPR) proteins were studied using Western blot analysis. Gene expression of UPR pathways was analyzed using rea.. View More»
  DOI: 10.4172/2167-0889.1000158

  Abstract PDF