Biochemistry & Analytical Biochemistry

Javaid Asad M

Publications

• Research Article
  Production, Purification and Characterization of Endopolygalacturonase by Bacillus subtillus
  Author(s): Nayab Munir, Javaid Asad M and Haidri SH Nayab Munir, Javaid Asad M and Haidri SH
  
  Plant cell wall is made up of complex carbohydrates like cellulose, hemicelluloses and pectin. Pectin is complex carbohydrates made up of galacturonic acid units linked by glycosidic linkage. For breakdown of this pectin microorganism require many different enzymes. Polygalacturonase is a pectinolytic enzyme catalyzed hydrolysis of α 1-4 glycosidic linkage in polygalacturonic acid by the addition of water. Polygalacturonase has two types; endopolygalacturonase and exopolygalacturonase. Endopolygalacturonase catalysis internal α 1-4 glycosidic linkage and exopolygalacturonase catalyze external α 1-4 glycosidic linkage in Pectate molecules. Endopolygalacturonase has various applications especially fruit juice industries used for clarification of juice as well as in food industry for maceration of vegetables. Endo-PG produced by fungus .. View More»
  DOI: 10.4172/2161-1009.1000181

  Abstract PDF