Journal of Nanomedicine & Nanotechnology

Jai Shankar

Publications

• Research Article
  Synthesis of Nanoparticles Using Euphorbia prostrata Extract Reveals a Shift from Apoptosis to G0/G1 Arrest in Leishmania donovani
  Author(s): Abdul Abduz Zahir, Indira Singh Chauhan, Asokan Bagavan, Chinnaperumal Kamaraj, Gandhi Elango, Jai Shankar, Nidhi Arjaria, Mohana Roopan, Abdul Abdul Rahuman and Neeloo SinghAbdul Abduz Zahir, Indira Singh Chauhan, Asokan Bagavan, Chinnaperumal Kamaraj, Gandhi Elango, Jai Shankar, Nidhi Arjaria, Mohana Roopan, Abdul Abdul Rahuman and Neeloo Singh
  
  The aim of the present investigation was to synthesize silver (Ag) and titanium dioxide (TiO2) nanoparticles (NPs) using the aqueous leaves extract of Euphorbia prostrata as antileishmanial agents and to explore the mechanism of induced cell death. In vitro antileishmanial activity of synthesized NPs was tested against promastigotes of Leishmania donovani by alamar Blue® cell viability reagent and propidium iodide uptake assay. The effective leishmanicidal activity of synthesized Ag NPs was further confirmed by cell cycle progression, externalized phosphatidylserine, DNA fragmentation assay, reactive oxygen species (ROS) level, intracellular non-protein thiols and transmission electron microscopy (TEM) of the treated parasites. TEM analysis of the synthesized Ag NPs and TiO2 NPs showed spherical shape with an average size of 12.82 ± 2.50 and 83.22 &plu.. View More»
  DOI: 10.4172/2157-7439.1000213

  Abstract PDF