Ingrid Reis da Silva
Microbiology Laboratory, Amazon Biotechnology Center, CBA and Biotechnology,
Manaus, Amazonas
Brazil
Research Article
The Effect of Varying Culture Conditions on the Production of Antibiotics by Streptomyces spp., Isolated from the Amazonian Soil
Author(s): Ingrid Reis da Silva, Mayra Kassawara Martins, Clarice Maia Carvalho, João Lúcio de Azevedo and Rudi Emerson de Lima Procópio
Ingrid Reis da Silva, Mayra Kassawara Martins, Clarice Maia Carvalho, João Lúcio de Azevedo and Rudi Emerson de Lima Procópio
The genus Streptomyces is considered to be of great industrial importance because of its ability to produce secondary metabolites that account for 80% of the antibiotics currently in use. To optimise the production of antimicrobial compounds from three strains of Streptomyces spp. isolated from the Amazon?s soil, we investigated the influence of physical (temperature, pH, agitation and time) and chemical (concentrations of carbon and nitrogen) variables, according to a factorial statistical design consisting of three repetitions at the central point. During a period between five and twenty days of incubation, the temperature was varied between 20 and 40°C, the pH was varied between 4.5 and 8.5, and the agitation was varied between 100 and 300 rpm. The concentrations of carbon and nitrogen sources ranged from 5 to 15 g/L and 0.5 to 1.5 g/L, respectively, and the results were evaluated usi.. View More»
DOI:
10.4172/2167-7972.1000105