Indu Sharma
Silchar-788011
India
Research Article
Detection of invA Gene in Isolated Salmonella from Marketed Poultry Meat by PCR Assay
Author(s): Indu Sharma and Kashmiri DasIndu Sharma and Kashmiri Das
Background: The aims of the current study were to detect the invA gene from chicken samples meant for human consumption from N.E India. Materials and method: After Salmonella sp. identification with culture method, PCR assay were developed for detection of pathogenic genes and antibiotic resistance genes of Salmonella sp. Results: Salmonella was detected in 80 samples of poultry carcasses from main poultry markets in Silchar, Assam, N.E India. A total of 40 Salmonella isolates were found in chicken samples (43%) and the isolates had a growth on brilliant green agar and De-oxycholate citrate agar medium, were oxidase negative and catalase positive and exhibited no changes in the colour of the medium with 100% motility. All the strains were subjected to Salmonella-specific gene (invA) and were confirmed as Salmonella positive by the predicted product of 284-.. View More»
DOI:
10.4172/2157-7110.1000564