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Ievgenia M Krasnobryzha
Ievgenia M Krasnobryzha
Ukraine
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Research Article
The Simultaneous Human FVIII/vWF Purification and Virus Inactivation Combined in Chromatographic Column
Author(s): Sergiy P Havryliuk, Ievgenia M Krasnobryzha, Olena S Havryliuk and Georgii L Volkov Sergiy P Havryliuk, Ievgenia M Krasnobryzha, Olena S Havryliuk and Georgii L Volkov
Well known that the decrease of the number of degrees of freedom prevents protein?s denaturation. In the previous investigation, we showed that the chromatographic gel binding protein can serves as an agent reducing the number of degrees of freedom of the protein. In addition, the high dynamic capacity of new developed gels provided a satisfactory retention of the peptide/protein at elevated temperature of the chromatographic process. These both factors allowed the implement of the virus inactivation for the peptide (streptokinase fragment SK1-61) and low (milk lysozyme) and an average (Fibrinogenolytic enzyme from the snake venom) molecular weight protein directly in the column during targets isolation. The goal of this study was an investigation of the possibility to save the activity of the high molecular weight complex FVIII/vWF, bound by ion exchange and affinity gels during the vir.. View More»
DOI: 10.4172/2167-7956.1000157