Hossein Rahmani
West Indies
Research Article
Growth Parameters Evaluation and Identification of Growth Hormone Receptor Gene Polymorphisms in Various Strains of Rainbow Trout Oncorhynchus mykiss
Author(s): Abdolvahab Ebrahimpour Gorji, Hossein Rahmani and Ghodrat Rahimi Miyanji
Abdolvahab Ebrahimpour Gorji, Hossein Rahmani and Ghodrat Rahimi Miyanji
In the present study, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and restriction fragment length polymorphism (RFLP) methods were compared to analyze the polymorphism of growth hormone receptor (GHR) gene in French, Iranian and Danish strains of Oncorhynchus mykiss. A monomorphic SSCP pattern of AA genotype in the French and Iranian strains and a dimorphic AA and AB genotype in the Danish strain were observed in 3' non-coding regions of GHR gene. In the Danish strain, the AB genotype polymorphism of the GHR gene with its very low frequency (5%) has no effect on the production trait. While, RFLP-Dde1 showed no polymorphism with genetic variation in the location of GHR. Moreover, comparing the condition factors (K) of three different rainbow trout strains showed a significant correlation between the.. View More»
DOI:
10.4172/2155-9546.1000435