Journal of Plant Pathology & Microbiology

Hossein Jafary

Publications

• Research Article
  Detection of Coat Protein Gene of the Potato Leafroll Virus by Reverse Transcription Loop-Mediated Isothermal Amplification
  Author(s): Mohammad Amin Almasi, Hossein Jafary, Aboubakr Moradi, Neda Zand, Mehdi Aghapour Ojaghkandi and Saeedeh AghaeiMohammad Amin Almasi, Hossein Jafary, Aboubakr Moradi, Neda Zand, Mehdi Aghapour Ojaghkandi and Saeedeh Aghaei
  
  Loop-mediated isothermal amplification assay amplifies DNA/RNA with high specificity and sensitivity. In this study, we describe an optimized reverse transcription- LAMP assay for detection of Potato Leafroll Virus. Firstly, DAS-ELISA assay was performed to detect of the virus in a collection containing 40 suspicious samples. Lastly, two samples were detected as the positive samples. Then, the positive samples were verified by RT-PCR and RT-LAMP methods. Furthermore, the results demonstrated that the RT-LAMP assay was 40 times sensitive and 4 time faster compared to RT-PCR. RT-LAMP assay was accomplished in the water bath either frees from any thermal cycler machine or sophisticated laboratories facility. Moreover, in RT-LAMP reaction the positive samples were detected through turbidity which produced by magnesium pyrophosphate. Interestingly, the application of CaCl2 instead of.. View More»
  DOI: 10.4172/2157-7471.1000156

  Abstract PDF