Journal of Aquaculture Research & Development

Hiroyuki Mizuta

Publications

• Research Article
  Identification and Efficient Utilization of Antibiotics for the Development of a Stable Transformation System in Porphyra yezoensis (Bangiales, Rhodophyta)
  Author(s): Megumu Takahashi, Koji Mikami, Hiroyuki Mizuta and Naotsune SagaMegumu Takahashi, Koji Mikami, Hiroyuki Mizuta and Naotsune Saga
  
  Despite the development of transient gene expression in red algae Porphyra species, a stable transformation system has not been stablished in these algae yet. One reason for the difficulty in the transformation is the lack of a way to select transformed cells from the algae. Thus, to identify antibiotics suitable for the selection of stably transformed cells, we tested the gametophyte for sensitivity to 6 antibiotics, ampicillin (Am), kanamycin (Km), hygromycin (Hm), geneticin (G418), chloramphenicol (Cm) and paromomycin (Pm), which are frequently employed as selection agents in the transformation of land plants and microalgae. Sensitivity tests, which were carried out with different concentrations (0-10 mg mL-1) of antibiotics, showed that Hm, G418, Cm and Pm exhibited a lethal effect at more than 1.0 mg mL-1, suggesting the suitability of these four antibiotics for the selection of .. View More»
  DOI: 10.4172/2155-9546.S3-002

  Abstract PDF