Journal of Nanomedicine & Nanotechnology

Hideki Aizaki

Publications

• Research Article
  Nanoimaging of ssRNA: Genome Architecture of the Hepatitis C Virus Revealed by Atomic Force Microscopy
  Author(s): Jamie L Gilmore, Hideki Aizaki, Aiko Yoshida, Katashi Deguchi, Masahiro Kumeta, Julia Junghof, Takaji Wakita and Kunio TakeyasuJamie L Gilmore, Hideki Aizaki, Aiko Yoshida, Katashi Deguchi, Masahiro Kumeta, Julia Junghof, Takaji Wakita and Kunio Takeyasu
  
  The complex structures that RNA molecules fold into play important roles in their ability to perform various functions in the cell. The structure and composition of viral RNA influences the ability of the virus to implement the various stages of the viral lifecycle and can influence the severity of the virus effects on the host. Although many individual secondary structures and some tertiary interactions of the Hepatitis C virus genome have previously been identified, the global 3D architecture of the full 9678 nucleotide genome still remains uncertain. One promising technique for the determination of the overall 3D structure of large RNA molecules is nanoimaging with Atomic Force Microscopy. In order to get an idea of the structure of the HCV genome, we imaged the RNA prepared in the presence of Mg2+, which allowed us to observe the compact folded tertiary structure of the viral geno.. View More»
  DOI: 10.4172/2157-7439.S5-010

  Abstract PDF