Hector Herrera
Chile
Review Article
Nucleotides and Effect Over Starving Condition on Fish SHK-1 Cells Model
Author(s): Pamela Olivares, Rodrigo Sanchez, Erico Carmona, Allisson Astuya, Hector Herrera and Jorge Parodi
Pamela Olivares, Rodrigo Sanchez, Erico Carmona, Allisson Astuya, Hector Herrera and Jorge Parodi
Additives play an important role in nutrition for boosting the physiological processes of productive species, and starvation has become a new problem for the industry. Only in recent years have studies began to analyze the effects of additives on fish cells. We observed the effects of nucleotides, which is a compound highly utilized in aquaculture. The SHK-1 cell line was derived from the head and kidney of Atlantic salmon as a biological model. Samples were exposed to nucleotides at incremental times and concentrations to determine the effects on cell viability by evaluating the number of cells. The cells were also exposed to a starving condition and recovery when nucleotides were used. Our data indicate that nucleotides are not sufficient to stimulate cellular proliferation. Cultures were exposed to a basal medium without serum in t.. View More»
DOI:
10.4172/2155-9546.1000448