Journal of Aquaculture Research & Development

Harresh Adikesavalu

Publications

• Research Article
  Polypeptide Profiling of Pangas Catfish (Pangasius pangasius) Serum Globulin Protein Fraction and Development of Anti-pangas Serum Globulin-HRPO Immunoconjugate for Rapid Detection of Bacterial Infection
  Author(s): Harresh Adikesavalu, Pradipta Paul, Siddhartha N Joardar and Jawahar T Abraham Harresh Adikesavalu, Pradipta Paul, Siddhartha N Joardar and Jawahar T Abraham
  
  Bacterial diseases continue to be a major economic factor for commercial catfish farming. Correct diagnosis of diseases is, therefore, important for effective treatment measures and to prevent production losses. The present study aimed at fractionating and characterizing the serum globulin proteins of pangas catfish, Pangasius pangasius by salt fractionation and SDS-PAGE analysis. The protein profile of fractionated serum globulin proteins of P. pangasius revealed a total of 12 bands, viz., 136.64, 120.58, 95.87, 79.92, 72.67, 63.04, 45.99, 43.32, 38.65, 34.82, 28.46 and 19.08 kDa. Anti-pangas serum globulin (ASG) horseradish peroxidase (HRPO) immunoconjugate (ASGHRPO immunoconjugate) was prepared following sodium periodate procedure and raising immune serum against it in rabbit. The sero-reactivity of ASG-HRPO immunoconjugate was assessed by dot-ELISA and direct ELIS.. View More»
  DOI: 10.4172/2155-9546.1000458

  Abstract PDF