Journal of Nanomedicine & Nanotechnology

Harinatha Reddy A

Publications

• Research Article
  Synthesis of Silk Silver Nanoparticles form Silkworm Cocoons and Their Antibacterial Activity on Methicillin Resistant Staphylococcus aureus (MRSA) and Escherichia coli
  Author(s): Harinatha Reddy A and Venkatappa BHarinatha Reddy A and Venkatappa B
  
  In this study, silk protein was used for the synthesis of silver nanoparticles (AgNPs). The freshly prepared silk colloid solution is color less, after 24 h incubation with AgNO3, the color change was observed from colourless to brown colour. The AgNPs were characterized by UV-visible spectrophotometer. The colloid solution of silk showed an absorption peak between 250-280 nm, after 24 h of incubation with AgNO3 the absorbance peak was centered near 420 nm indicating the reduction of silver nitrate into silver nanoparticles by silk protein. Bacterial sensitivity to silver nanoparticles is commonly tested using a well diffusion assay. MRSA and E. coli were isolated from hospital soil samples. S. aureus and E. coli identified by with by microscopic and biochemical tests. MRSA identified in the selected isolates by the Kirby- Bauer disk diffusion test with vancomycin (30 μg), ampicill.. View More»
  DOI: 10.4172/2157-7439.1000453

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