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Hafez EE
Hafez EE
City for Scientific Research and Technology Applications, Arid Lands Cultivations Research Institute,
New Borg El-Arab, 21934, Alexandria
Egypt
Publications
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Research Article
Induction of New Defensin Genes in Tomato Plants via Pathogens-Biocontrol Agent Interaction
Author(s): Hafez EE, Hashem M, Mahmoud M Balbaa, El-Saadani MA and Seham A AhmedHafez EE, Hashem M, Mahmoud M Balbaa, El-Saadani MA and Seham A Ahmed
Defensins and defensin-like peptides are functionally diverse and commonly presented as an immune reaction between plant and pathogen. Trichoderma viride and Bacillus subtilis as biological control agents, were inoculated into the soil, to suppress the activity of the pathogenic fungi Fusarium oxysporum and Rhizoctonia solani on tomato. The up- and down–regulated genes were examined in both treated and non-treated plants, using differential display technique. In treated plants, many up-regulated genes (21) with different molecular sizes, ranging from 50 to 7000 bp, were observed. Only four up-regulated genes were isolated from plants treated with B. subtilis+R. solani. The sequence analysis revealed that the identified genes were defensin genes; Amino Acid/Auxin Permease Family, Endopolygalacturonase PG1, Fructose-1,6-bisphosphatase and glycosyl transferase. Moreover, chi.. View More»
DOI: 10.4172/2157-7471.1000167