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Habib Abdul Hakim Esa
Habib Abdul Hakim Esa
Infectious Disease Research Centre, Institute for Medical Research,
Jalan Pahang, 50588 Kuala Lumpur
Malaysia
Publications
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Research Article
Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay Kit for Detection of IgM Antibody against Leptospira in Human Sera for Patients admitted in Malaysian Hospitals
Author(s): Habib Abdul Hakim Esa*, Fairuz Amran and Nurul Atiqah Noor Halim Habib Abdul Hakim Esa*, Fairuz Amran and Nurul Atiqah Noor Halim
Leptospirosis is an infectious zoonotic disease caused by corkscrew shaped of pathogenic Leptospira spp. Early diagnosis is important as the disease can be treated with antibiotic to prevent complications. The gold standard of leptospirosis diagnosis is microscopic agglutination test (MAT). However, the test is laborious and time consuming. This study aimed to evaluate whether a commercial ELISA (enzyme-linked immunosorbent assay) kit by Virion- Serion can demonstrate antibody against leptospires for accurate and rapid screening for leptospirosis. A total of 212 serum samples were tested with Virion-Serion Classic ELISA IgM, which was earlier collected from patient with clinical manifestation suggestive of leptospirosis in Malaysia and confirmed with MAT. The results were analyzed into two categories. In the first category all samples that were positive with MAT but detected as interm.. View More»
DOI: 10.4172/2327-5073.1000307